Database error: Invalid SQL: select count(id) from pwn_comment where pid='140581' and iffb='1'
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select count(id) from pwn_comment where pid='140581' and iffb='1') called at [/virtualhost/YJCOM2upmxT/includes/db.inc.php:65] #1 dbbase_sql->query(select count(id) from {P}_comment where pid='140581' and iffb='1') called at [/virtualhost/YJCOM2upmxT/comment/module/CommentContent.php:65] #2 CommentContent() called at [/virtualhost/YJCOM2upmxT/includes/common.inc.php:524] #3 PrintPage() called at [/virtualhost/YJCOM2upmxT/comment/html/index.php:13] Database error: Invalid SQL: select * from pwn_comment where pid='140581' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='140581' and iffb='1' order by id limit 0,10) called at [/virtualhost/YJCOM2upmxT/includes/db.inc.php:65] #1 dbbase_sql->query(select * from {P}_comment where pid='140581' and iffb='1' order by id limit 0,10) called at [/virtualhost/YJCOM2upmxT/comment/module/CommentContent.php:167] #2 CommentContent() called at [/virtualhost/YJCOM2upmxT/includes/common.inc.php:524] #3 PrintPage() called at [/virtualhost/YJCOM2upmxT/comment/html/index.php:13] 网友点评-Tion machinery inside of evolutionarily distant hosts. Exploitation of the polyubiquitination machinery-家电商城
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发布于:2019-7-11 17:57:03  访问:18 次 回复: 篇
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Tion machinery inside of evolutionarily distant hosts. Exploitation of the polyubiquitination machinery
The reduce in Adriamycin (hydrochloride) price translocation may well be thanks to poor folding on the Doxorubicin custom synthesis protein to become presented for passage by way of the Dot/Icm channel, or the ANK domains might have distinct translocation alerts that aid in export of AnkB by means of the Dot/Icm system. Our details exclude the likelihood which the minimized translocation on the truncated variants of AnkB is liable for your practical defect, which relies on quite a few lines of evidence. Initially, ectopic expression with the AnkB A1, AnkB A2, or AnkB A1A2 truncated variants in mammalian cells leads to failure to focus on the truncated variant proteins into the host plasma membrane, while the full-length AnkB is especially focused on the plasma membrane. 2nd, even though ectopically expressed full-length AnkB recruits polyubiquitinated proteins to your plasma membrane, the AnkB A1, AnkB A2, or AnkB A1A2 truncated variants fail to exhibit the identical process. Third, ectopic expression from the WT AnkB trans-rescues the ankB mutant for its defect in intracellular proliferation even though the AnkB A1, AnkB A2 or AnkB A1A2 truncated variants fall short to trans-rescue the ankB null mutant for its defect in intracellular proliferation. Taken jointly, we conclude the two ANK domains of AnkB are essential for its perform in decoration of the LCV with polyubiquitinated proteins and for intracellular proliferation. Whilst most F-box proteins generally have an LRR or WD protein-protein conversation area, the ANK protein-protein conversation domains have not been uncovered in eukaryotic Fbox proteins (23, 24, 36, forty two). This indicates a noncanonical framework for your AnkB F-box protein in contrast to eukaryotic F-box proteins. When the F-box area of AnkB is essential for conversation with the host Skp1 part of the SCF1 ubiquitin ligase, the two ANK domains are possible concerned in binding to substrates specific for polyubiquitination and linking them to PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/21034305 the SCF1 ubiquitin ligase intricate sure on the F-box domain to induce their polyubiquitination. Considering the fact that AnkB is targeted to PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24021036 host membranes, it is actually much more very likely that many of the targets of AnkB are localized to or involved with host mobile membranes. It is very likely that several of the substrates targeted byAnkB are highly conserved in mammalian and protozoan cells, for the reason that two ANK domains are important for the perform of AnkB in the two hosts.Tion equipment within just evolutionarily distant hosts. Exploitation on the polyubiquitination machinery is the first illustration of manipulation of conserved eukaryotic processes by L. pneumophila within just mammalian and protozoan cells. Deletion of possibly with the two ANK domains abolishes recruitment of polyubiquitinated proteins for the LCV in just macrophages and amoebae. Moreover, deletion of possibly of the two ANK domains fails to complement the ankB null mutant for its defect in intracellular replication. Having said that, deletion of both or both of those on the ANK domains results in a decrease in export of your truncated proteins from the Dot/Icm system. The minimize in translocation might be because of to improper folding of the protein to generally be offered for passage through the Dot/Icm channel, or perhaps the ANK domains could possibly have specific translocation alerts that aid in export of AnkB by the Dot/Icm process.
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